Sensitive detection of S. Aureus using aptamer- and vancomycin -copper nanoclusters as dual recognition strategy

The proposed aptamer- and antibiotic-based dual detection sensor, combines copper nanoclusters (CuNCs) as an effective approach for the recognition and quantification of Staphylococcus aureus (S. aureus) as a pathogenic bacteria. A facile method for CuNCs based on vancomycin as the template using a fluorescence platform was proposed for the recognition of the S. aureus whole cells via antibiotic and aptamer. Using dual receptor functionalized CuNCs linked to vancomycin and a specific aptamer and during aggregation induce emission process enhanced fluorescence signal linearly with S. aureus concentrations between 10²-10⁸ CFU/mL, and the detection limit was 80 CFU/mL after 45 min as the optimum incubation time. Non-target bacteria generated negative results, proving the high specificity of the presented sensor. This strategy showed recoveries ranging 86%-98% in milk as real sample and can be used for the development of universal detection platforms for efficient and specific S. aureus detection with great potential applications for monitoring pathogenic bacteria.