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Updated: Nov 4, 2025

Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA
Published on: May 2, 2018
Elemental and microbiota content in indoor and outdoor air using recuperation unit filters
Naděžda Zíková1, Zbigniew Ziembik2, Tomasz Olszowski3
1Institute for Environmental Studies, Faculty of Science, Charles University in Prague, Albertov 6, 128 43 Prague, Czech Republic; Department of Aerosol Chemistry and Physics, Institute of Chemical Process Fundamentals of the Czech Academy of Sciences, Rozvojová 1/135, 165 02 Prague, Czech Republic.
Abstract:
This paper presents the results of a twelve-month measurement campaign conducted at a rural single-family house in Poland. The external and internal filters of a recuperator used to mechanically ventilate the building were used to separate the total suspended particles (TSPs), and the concentrations of fifteen elements and abundance of fungi and bacteria were determined. Lower annual mean concentrations were observed indoors, and the concentrations of most elements did not significantly change between seasons. There were some differences between winter and summer, which may have resulted from changes in the ventilation regimes in the house. The number of bacteria was similar outdoors and indoors, while the amounts of fungi were higher indoors (p < 0.05). The order of metal concentrations outdoors agreed well with observations in other countries, while indoors the metal concentrations order indicated the individual characteristics of the building. The species diversity of fungi was higher than that of bacteria, and different species were found indoors and outdoors, while bacteria were typically present both indoors and outdoors. Different TSP sources were identified indoors and outdoors, suggesting limited penetration between the two environments. However, both environments were affected by traffic. Mechanical ventilation systems with built-in filters (such as recuperators) were useful in assessing the air quality within the building, and the changeable recuperation filters offer an approach to assess the air quality in several houses without any additional cost or discomfort to the residents.
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