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Fast SARS-CoV-2 virus detection using disposable cartridge strips and a semiconductor-based biosensor platform.
Minghan Xian1, Hao Luo1, Xinyi Xia1
1Department of Chemical Engineering, University of Florida, Gainesville, Florida 32611.
Journal of Vacuum Science and Technology. B, Nanotechnology & Microelectronics : Materials, Processing, Measurement, & Phenomena : JVST B
|May 31, 2021
Summary
A new biosensor detects SARS-CoV-2 spike protein and virus using functionalized strips and a metal-oxide-semiconductor field-effect transistor (MOSFET). This portable, low-cost system offers potential for rapid point-of-care viral disease diagnostics.
Area of Science:
- Biosensor technology
- Nanomaterials
- Infectious disease diagnostics
Background:
- Accurate and rapid detection of SARS-CoV-2 is crucial for managing viral outbreaks.
- Existing diagnostic methods can be time-consuming and require specialized laboratory equipment.
- Development of portable, cost-effective diagnostic tools is essential for point-of-care applications.
Purpose of the Study:
- To develop a novel biosensor for the detection of SARS-CoV-2.
- To integrate biofunctionalized sensor strips with a reusable printed circuit board and MOSFET for signal amplification.
- To evaluate the sensor's performance for detecting viral proteins and inactivated virus particles.
Main Methods:
- Biofunctionalization of disposable sensor strips with monoclonal and polyclonal antibodies.
- Immobilization of antibodies onto gold-plated electrodes via thiol group binding.
- Verification of biofunctionalization using scanning electron microscopy and electrical measurements.
- Integration of sensor strips with a MOSFET for signal amplification and readout.
- Application of synchronous pulses and analysis of drain waveform dynamics.
Main Results:
- Successful immobilization of antibodies and formation of gold clusters on sensor strips confirmed.
- Detection of SARS-CoV-2 spike protein and inactivated virus achieved.
- Broad detection range for proteins (1 fg/ml to 10 μg/ml) and virus (100 to 2500 PFU/ml).
- Correlation between signal output and analyte concentration established.
Conclusions:
- The developed biosensor demonstrates high sensitivity and a wide dynamic range.
- The system shows potential for portable, low-cost, disposable cartridge-based point-of-care diagnostics.
- This approach could be valuable for the rapid detection of various viral diseases.
