Pregnancy resulting from cryopreserved human embryos using a one-step in situ dilution procedure

In vitro fertilization and embryo transfer require the use of hormonal manipulation and surgery that may reduce the receptivity of the patient's uterus during the stimulated cycle. Cryopreservation of human embryos eliminates the need for immediate transfer, permitting them to be stored until they can be transferred during subsequent unstimulated cycles. Embryo cryopreservation is an established procedure in the breeding of laboratory and domestic animals, but has only recently been applied to humans. We report on a pregnancy using a simple cryopreservation procedure that permits embryos to be diluted out of the cryoprotectant solution without removing them from the plastic straw in which they were cryopreserved.