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Nitrate and nitrite pathways and dynamic changes in bacterial communities during beet sugar processing.

Shuanghong Zhang1, Ziyi Wu1, Jian Wang1

  • 1Beijing Key Laboratory of Nutrition & Health and Food Safety, Nutrition and Health Research Institute Co. Ltd, Beijing, China.

Journal of the Science of Food and Agriculture
|May 31, 2021
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Bacterial communities in sugar beet processing significantly impact nitrate and nitrite levels. This study reveals their role in nitrogen metabolism, aiding manufacturers in controlling these compounds.

Keywords:
bacterial communitiesbeet sugardiffusion juicenitrate and nitrite

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Area of Science:

  • Microbiology
  • Food Science
  • Biochemistry

Background:

  • Bacterial community succession during sugar production was investigated.
  • The correlation between bacterial communities and nitrate/nitrite content in beet sugar processing was examined.

Purpose of the Study:

  • To survey bacterial communities throughout sugar beet processing stages.
  • To investigate the relationship between these communities and nitrate/nitrite levels.
  • To understand the role of bacteria in nitrogen compound transformation during sugar production.

Main Methods:

  • High-throughput sequencing of the V3-V4 region of the 16S rDNA gene was employed.
  • Analysis included samples from various processing stages: sugar beet, cossettes, diffusion juice, light juice, and thick juice.
  • Bacterial nitrogen metabolic potential was assessed, including a shaking experiment to quantify nitrate reduction.

Main Results:

  • Dominant bacterial genera identified were Pantoea, Pseudomonas, Leuconostoc, and Burkholderia.
  • Significant bacterial community shifts were observed across processing stages.
  • Bacterial communities demonstrated the capacity for nitrate and nitrite metabolism, with nitrate levels declining significantly (73% and 98%) in diffusion juices within 36 hours.

Conclusions:

  • Bacterial communities play a crucial role in the formation and transformation of nitrate and nitrite during beet sugar processing.
  • The study provides insights into bacterial pathways involved in nitrate/nitrite metabolism.
  • Findings can guide beet sugar manufacturers in managing and controlling nitrate and nitrite content.