Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Nov 4, 2025

Nuclear Migration in the Drosophila Oocyte
04:17

Nuclear Migration in the Drosophila Oocyte

Published on: May 13, 2021

4.2K

Nuclear Migration in the Drosophila Oocyte.

Maëlys Loh1, Antoine Guichet2, Fred Bernard3

  • 1Université de Paris, CNRS, Institut Jacques Monod.

Journal of Visualized Experiments : Jove
|May 31, 2021
PubMed
Summary

Live cell imaging is crucial for understanding nuclear migration in Drosophila oocytes. This study presents a new protocol for long-term live imaging of egg chambers, enabling visualization of 3D nuclear positioning.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Girdin controls the pace of 3D tracheal cell intercalation by coupling adherens junctions to the actin cytoskeleton in Drosophila.

Development (Cambridge, England)·2026
Same author

JAK-STAT-dependent contact between follicle cells and the oocyte controls Drosophila anterior-posterior polarity and germline development.

Nature communications·2024
Same author

The Importance of the Position of the Nucleus in <i>Drosophila</i> Oocyte Development.

Cells·2024
Same author

Multifaceted modes of γ-tubulin complex recruitment and microtubule nucleation at mitotic centrosomes.

The Journal of cell biology·2023
Same author

Kinesin-1 promotes centrosome clustering and nuclear migration in the Drosophila oocyte.

Development (Cambridge, England)·2023
Same author

Basal stem cell progeny establish their apical surface in a junctional niche during turnover of an adult barrier epithelium.

Nature cell biology·2023

Area of Science:

  • Developmental Biology
  • Cell Biology
  • Genetics

Background:

  • Live cell imaging is essential for studying dynamic cellular processes like organelle movement and cytoskeleton rearrangements.
  • Oocyte nucleus positioning is critical for establishing embryonic polarity, with Drosophila serving as a key model system.
  • Nuclear migration in Drosophila oocytes occurs in three dimensions and is driven by microtubule forces.

Purpose of the Study:

  • To develop and validate a protocol for long-term live imaging of Drosophila egg chambers.
  • To enable visualization of the dynamic 3D migration of the oocyte nucleus.
  • To facilitate the study of molecular mechanisms regulating nuclear positioning.

Main Methods:

  • Dissection of Drosophila egg chambers.

More Related Videos

Upright Imaging of Drosophila Egg Chambers
12:29

Upright Imaging of Drosophila Egg Chambers

Published on: March 13, 2015

7.9K
Imaging Through the Pupal Case of Drosophila melanogaster
06:50

Imaging Through the Pupal Case of Drosophila melanogaster

Published on: January 23, 2014

13.5K

Related Experiment Videos

Last Updated: Nov 4, 2025

Nuclear Migration in the Drosophila Oocyte
04:17

Nuclear Migration in the Drosophila Oocyte

Published on: May 13, 2021

4.2K
Upright Imaging of Drosophila Egg Chambers
12:29

Upright Imaging of Drosophila Egg Chambers

Published on: March 13, 2015

7.9K
Imaging Through the Pupal Case of Drosophila melanogaster
06:50

Imaging Through the Pupal Case of Drosophila melanogaster

Published on: January 23, 2014

13.5K
  • Culture of dissected egg chambers for extended periods (up to 12 hours).
  • Time-lapse spinning-disk confocal microscopy for 3D live imaging.

    • Main Results:

    • The developed protocol successfully preserves Drosophila egg chambers, allowing them to remain viable for 12 hours.
    • The protocol enables visualization of the complete 3D nuclear migration process within the oocyte.
    • This method facilitates the study of nuclear positioning dynamics in a large number of samples.

    Conclusions:

    • The established live-imaging protocol is effective for studying 3D nuclear migration in Drosophila oocytes.
    • This technique provides a valuable tool for investigating the mechanisms controlling nuclear positioning and its role in establishing polarity.
    • The protocol supports long-term observation, crucial for capturing complex dynamic events in developmental biology.