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Updated: Nov 3, 2025

Live Imaging of the Mitochondrial Glutathione Redox State in Primary Neurons using a Ratiometric Indicator
Published on: October 20, 2021
Assessment of Mitochondrial Protein Glutathionylation as Signaling for CO Pathway
Ana S Almeida1, Cláudia Figueiredo-Pereira1, Helena L A Vieira2,3,4
1CEDOC, Faculdade de Ciência Médicas/NOVA Medical School, Universidade Nova de Lisboa, Lisboa, Portugal.
Abstract:
Protein glutathionylation is a posttranslational process that regulates protein function in response to redox cellular changes. Furthermore, carbon monoxide-induced cellular pathways involve reactive oxygen species (ROS) signaling and mitochondrial protein glutathionylation. Herein, it is described as a technique to assess mitochondrial glutathionylation due to low concentrations of CO exposure. Mitochondria are isolated from cell culture or tissue, followed by an immunoprecipitation assay, which allows the capture of any glutathionylated mitochondrial protein using a specific antibody coupled to a solid matrix that binds to glutathione antigen. The precipitated protein is further identified and quantified by immunoblotting analysis.
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