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EPR Monitored Redox Titration of the Cofactors of Saccharomyces cerevisiae Nar1
Published on: November 26, 2014
Direct electrochemical probes of redox protein and redox enzyme structure and function
1Inorganic Chemistry Laboratory, Oxford University, UK.
Abstract:
The direct electrochemistry of redox proteins has been achieved at a variety of electrodes, including modified gold and pyrolytic graphite. Careful design of electrode surfaces and electrolyte conditions are required for the attainment of rapid and reversible protein-electrode interaction. Protein-protein complexes can be studied by using selective electrode surfaces. The "well-behaved" electrochemistry of redox proteins can be exploited by coupling the electrode reaction to enzymes for which the redox proteins act as cofactors. The electron-transfer reactions of more complex systems, such as redox enzymes, are now being examined.
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