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Design Example01:23

Design Example

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The innovation of touch-tone telephony revolutionized the telecommunications industry by replacing the traditional rotary dial with a dual-tone multi-frequency (DTMF) signaling system. This system uses a matrix-style keypad with buttons arranged in four rows and three columns, creating 12 distinct signals each assigned to a pair of frequencies. Each button press results in a simultaneous generation of two sinusoidal tones – one from a low-frequency group (697 to 941 Hz) and one from a...
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Design Consideration01:22

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Designing a structure involves a series of considerations, primarily the material's ultimate strength, calculated through tests that measure changes under increased force until the material reaches its breaking point or limit. The ultimate load, where the material breaks, is divided by its original cross-sectional area, resulting in the ultimate normal stress or strength. The ultimate shearing stress is another significant factor taken into account.
The factor of safety is another key...
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Data are individual items of information obtained from a population or sample. Data may be classified as qualitative (categorical), quantitative continuous, or quantitative discrete. Because it is not practical to measure the entire population in a study, researchers use samples to represent the population. A random sample is a representative group from the population chosen by using a method that gives each individual in the population an equal chance of being included in the sample. Random...
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In statistics, several tools are used to interpret the data. Measures of central tendency represent the characteristics of the data, such as mean, median, and mode. Additionally, measures of variance like standard deviation and range are used to find the spread of data from the mean. Relative standing measures the distance between data locations. Commonly used measures of relative standings are percentile, z score, and quartiles.
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The design of prismatic beams, structural elements with a uniform cross-section, focuses on ensuring safety and structural integrity under load. The design process begins by determining the allowable stress, either from material properties tables, or by dividing the material's ultimate strength by a safety factor. This safety factor is essential for accommodating uncertainties, and varies depending on the material—timber, steel, or concrete—with each having unique strength and...
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Design Example: Managing Concrete Workability01:14

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This example deals with managing the workability of concrete for a raft foundation project under hot weather conditions. Workability is crucial for ensuring the concrete is easy to place, compact, and finish. In this scenario, a slump test — a common method to measure the workability of fresh concrete — initially indicated low workability. This was attributed to the rapid water loss from the concrete mix, exacerbated by the high temperatures causing the course aggregates to heat up.
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pegIT - a web-based design tool for prime editing.

Mads Valdemar Anderson1, Jakob Haldrup1, Emil Aagaard Thomsen1

  • 1Department of Biomedicine, Aarhus University, Aarhus C, 8000, Denmark.

Nucleic Acids Research
|June 1, 2021
PubMed
Summary
This summary is machine-generated.

Prime editing (PE) enables precise genome editing without DNA breaks using prime editing guide RNAs (pegRNAs). The new pegIT tool simplifies rapid pegRNA design for various applications, improving efficiency.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Bioinformatics

Background:

  • Prime editing (PE) is an advanced CRISPR-derived genome editing technology.
  • PE enables precise DNA modifications without inducing double-stranded DNA breaks.
  • The technique relies on a Cas9-reverse transcriptase fusion protein and prime editing guide RNAs (pegRNAs).

Purpose of the Study:

  • To address the challenges of manual pegRNA design, which is prone to errors and unsuitable for large-scale applications.
  • To introduce pegIT, a user-friendly web tool for efficient and rapid pegRNA design.
  • To support numerous user-defined edits, including those required for large-scale genome editing projects.

Main Methods:

  • Development of a web-based tool named pegIT.
  • Implementation of algorithms for rapid design of dual-functioning prime editing guide RNAs (pegRNAs).
  • Facilitation of user-defined edits for various genome editing applications.

Main Results:

  • pegIT provides a user-friendly interface for designing pegRNAs.
  • The tool enables rapid and accurate design of pegRNAs for diverse editing needs.
  • pegIT supports both small-scale and large-scale genome editing setups.

Conclusions:

  • Manual pegRNA design is a bottleneck for efficient prime editing.
  • The pegIT web tool significantly streamlines the pegRNA design process.
  • pegIT is a valuable resource for researchers utilizing prime editing technology for precision genome engineering.