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Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Updated: Nov 3, 2025

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

Point-of-Care PCR Assays for COVID-19 Detection.

Niharika Gupta¹, Shine Augustine¹, Tarun Narayan²

¹Department of Biotechnology, Delhi Technological University, Shahbad Daulatpur, Delhi 110042, India.

|June 2, 2021

Summary

This summary is machine-generated.

Molecular diagnostics, including RT-PCR, are crucial for COVID-19 detection. Miniaturized PCR systems and point-of-care (POC) devices offer faster results and aid in mass screening efforts.

Keywords:

COVID-19 digital PCR electrochemical point-of-care polymerase chain reaction

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Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

Published on: March 31, 2021

Area of Science:

Molecular Diagnostics
Infectious Disease Detection
Biotechnology

Background:

Molecular diagnostics, particularly RT-PCR, established the gold standard for COVID-19 diagnosis.
Point-of-care (POC) devices and antigen tests significantly contributed to mass screening and rapid SARS-CoV-2 detection.
The limitations of traditional assays spurred interest in advanced diagnostic technologies.

Purpose of the Study:

To review recent advancements in miniaturized PCR systems for COVID-19 detection.
To explore novel technologies like digital PCR, electrochemical PCR, and CRISPR/Cas for POC diagnostics.
To discuss the impact and challenges of these emerging technologies.

Main Methods:

Literature review of recent developments in miniaturized PCR systems.
Analysis of digital PCR and electrochemical PCR technologies.
Evaluation of CRISPR/Cas applications in POC diagnostics.
Discussion of commercially available RT-PCR POC systems.

Main Results:

Miniaturized, high-throughput, and automated PCR systems are being developed for POC applications.
Digital PCR and electrochemical PCR offer distinct advantages and face specific challenges.
CRISPR/Cas technology shows significant potential for rapid POC diagnostics.
Several commercial RT-PCR POC systems have received regulatory approval for COVID-19 detection.

Conclusions:

Advancements in miniaturized PCR and POC technologies are crucial for future pandemic preparedness.
These technologies enhance diagnostic accessibility, speed, and throughput.
Continued innovation in molecular diagnostics is vital for global health security.