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Updated: Nov 3, 2025
Fabrication of Size-Controlled and Emulsion-Free Chitosan-Genipin Microgels for Tissue Engineering Applications
Published on: April 13, 2022
Combined Analytical Approaches to Standardize and Characterize Biomaterials Formulations: Application to
Sofia Magli1,2, Lorenzo Rossi1,2, Cesare Consentino3
1Department of Biotechnology and Biosciences, University of Milano-Bicocca, 20126 Milan, Italy.
Abstract:
A protocol based on the combination of different analytical methodologies is proposed to standardize the experimental conditions for reproducible formulations of hybrid hydrogels. The final hybrid material, based on the combination of gelatin and chitosan functionalized with methylfuran and cross-linked with 4-arm-PEG-maleimide, is able to mimic role, dynamism, and structural complexity of the extracellular matrix. Physical-chemical properties of starting polymers and finals constructs were characterized exploiting the combination of HP-SEC-TDA, UV, FT-IR, NMR, and TGA.
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