Functionalization with a Polyphenol-Rich Pomace Extract Empowers a Ceramic Bone Filler with In Vitro Antioxidant,...

Area of Science:

• Biomaterials Science
• Periodontology
• Regenerative Medicine

Background:

• Periodontitis and oral diseases pose a significant global health challenge, linked to systemic conditions and dental implant complications.
• Current treatments aim to mitigate inflammatory bone loss and prevent implant failure, but regenerative strategies are needed.
• Functionalizing biomaterials with anti-inflammatory and antioxidant agents offers a promising avenue for periodontal tissue regeneration.

Purpose of the Study:

• To introduce and characterize a novel ceramic biomaterial, Synergoss Red (SR), functionalized with a polyphenol mixture from grape pomace.
• To evaluate the cytocompatibility and biological response of SR in vitro.
• To assess the potential of SR for periodontal tissue regeneration.

Main Methods:

• Synergoss Red (SR) biomaterial synthesized and functionalized with polyphenols from Croatina grape pomace.
• Comprehensive characterization of polyphenol extract (HPLC) and biomaterial (surface, composition).
• In vitro assays to assess cytocompatibility and biological responses in macrophages and osteoblast-like cells.

Main Results:

• SR demonstrated significant anti-inflammatory effects by downregulating inflammation in macrophages.
• SR stimulated osteoblast-like cells, promoting gene expression for early bone matrix deposition.
• SR mitigated bone remodeling by favorably altering the RANKL/OPG ratio.

Conclusions:

• Synergoss Red (SR) exhibits excellent cytocompatibility and beneficial effects on bone regeneration.
• The anti-inflammatory and antioxidant properties of its functionalizing polyphenols contribute to its regenerative potential.
• SR represents an innovative biomaterial for periodontal regenerative therapy and dental implant applications.