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Survival and Proliferation under Severely Hypoxic Microenvironments Using Cell-Laden Oxygenating Hydrogels
Shabir Hassan1, Berivan Cecen1, Ramon Peña-Garcia2,3
1Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA.
Journal of Functional Biomaterials
|June 2, 2021
Summary
Engineered tissues often lack oxygen, causing cell death. This study developed slow-releasing oxygen microparticles (OMPs) using calcium peroxide (CPO) in polycaprolactone (PCL) to improve engineered tissue survival and proliferation.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Engineered living tissues require adequate nutrient supply, particularly oxygen, to prevent necrosis and ensure implant success.
- Chronic hypoxia or anoxia in engineered tissues leads to poor neovascularization and clinical outcomes.
- Existing oxygen-generating biomaterials face challenges related to oxygen production rate and harmful reactive oxygen species (ROS).
Purpose of the Study:
- To develop a novel oxygen-releasing microparticle (OMP) system for enhanced engineered tissue survival.
- To control oxygen release kinetics and minimize detrimental effects of reactive oxygen species (ROS).
- To optimize OMP concentration for sustained oxygen delivery in tissue engineering scaffolds.
Main Methods:
- Calcium peroxide (CPO) was encapsulated within polycaprolactone (PCL) to create oxygen-releasing microparticles (OMPs).
- OMPs were incorporated into gelatin methacryloyl (GelMA) hydrogels.
- GelMA hydrogels with OMPs were used to culture encapsulated skeletal myoblasts, optimizing OMP concentration for sustained oxygen delivery.
Main Results:
- Polycaprolactone (PCL) encapsulation enabled slow hydrolysis of calcium peroxide (CPO), ensuring sustained oxygen release.
- The developed OMPs provided prolonged oxygen delivery over one week within GelMA hydrogels.
- Encapsulated skeletal myoblasts demonstrated improved survival and proliferation in the presence of optimized OMP concentrations.
Conclusions:
- The PCL-encapsulated CPO microparticle system offers a controlled and sustained oxygen delivery platform for engineered tissues.
- This technology has the potential to improve cell survival in ischemic conditions and enhance the success of artificial tissue implants.
- The developed oxygen releasing and delivery platform opens avenues for cell-based therapies and clinical translation for ischemic diseases.
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