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Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
Published on: March 1, 2013
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Peptide-Based Nanoparticles for Therapeutic Nucleic Acid Delivery.
Prisca Boisguérin1, Karidia Konate1, Emilie Josse1
1PhyMedExp, University of Montpellier, INSERM U1046, CNRS UMR 9214, 34295 Montpellier, France.
Biomedicines
|June 2, 2021
Summary
Cell-penetrating peptides (CPPs) are emerging as effective non-viral vectors for delivering therapeutic nucleic acids (NAs). This review highlights peptide-based nanoparticles (PBNs) for NA delivery, focusing on their formulation and characteristics.
Area of Science:
- Biotechnology
- Nanomedicine
- Molecular Biology
Background:
- Gene therapy utilizes therapeutic nucleic acids (NAs) for treating diseases.
- Delivering exogenous NAs remains a significant challenge in NA-based therapies.
- Cell-penetrating peptides (CPPs) are increasingly recognized as promising non-viral vectors for NA delivery.
Purpose of the Study:
- To review the biophysical and biological aspects of peptide-based nanoparticles (PBNs) for therapeutic NA delivery.
- To present relevant peptides and peptide families forming PBNs with NAs since 2015.
- To outline common features of effective PBNs for NA delivery.
Main Methods:
- Literature review of peptide-based nanoparticles (PBNs) for NA delivery.
- Analysis of formulation conditions and properties of CPPs/NAs.
- Characterization of PBNs for size, dispersity, and charge.
Main Results:
- CPPs form various nanocomplexes with NAs, creating PBNs.
- Relevant peptides and peptide families forming PBNs since 2015 are presented.
- Effective PBNs for in vitro and in vivo NA delivery share common features.
Conclusions:
- PBNs offer a versatile approach for therapeutic NA delivery.
- Defined formulation conditions yield PBNs with optimal characteristics (60-150 nm, PdI < 0.3, +10 to +40 mV charge).
- PBNs are a viable non-viral vector system for advancing gene therapy applications.
