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Related Experiment Video

Updated: Jun 11, 2026

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform
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Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

Published on: February 25, 2021

Formulation of Peptide-Based Nanoparticles Using a Microfluidic Device.

Thania Hammoum1, Karidia Konate1, Yannick Mousli2

  • 1PhyMedExp, University of Montpellier, INSERM U1046, CNRS UMR9214, Montpellier, France.

Journal of Peptide Science : an Official Publication of the European Peptide Society
|June 10, 2026
PubMed
Summary
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Peptide-based nanoparticles (PBN) offer a robust alternative for nucleic acid delivery. WRAP5-PBN demonstrated consistent size and potent gene silencing/expression, independent of formulation parameters, highlighting their scalability.

Area of Science:

  • Biotechnology
  • Nanomedicine
  • Drug Delivery

Background:

  • Lipid nanoparticles (LNPs) are common for nucleic acid delivery but require strict formulation control.
  • Peptide-based nanoparticles (PBNs) present a promising alternative with potentially simpler formulation processes.

Purpose of the Study:

  • To evaluate the formulation of WRAP5-based PBNs using microfluidics.
  • To assess the impact of process parameters (flow rate ratio, total flow rate, mixing channel) on PBN characteristics.
  • To determine the stability and biological activity of WRAP5-PBNs for nucleic acid delivery.

Main Methods:

  • Microfluidic device used for formulating 72 different WRAP5-PBN formulations.
  • Dynamic light scattering (DLS) for nanoparticle size and polydispersity index (PdI) analysis.
Keywords:
WRAP5microfluidicpDNApeptide‐based nanoparticlesiRNA

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Flash NanoPrecipitation for the Encapsulation of Hydrophobic and Hydrophilic Compounds in Polymeric Nanoparticles
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Last Updated: Jun 11, 2026

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform
09:41

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

Published on: February 25, 2021

Rapid, Scalable Assembly and Loading of Bioactive Proteins and Immunostimulants into Diverse Synthetic Nanocarriers Via Flash Nanoprecipitation
06:57

Rapid, Scalable Assembly and Loading of Bioactive Proteins and Immunostimulants into Diverse Synthetic Nanocarriers Via Flash Nanoprecipitation

Published on: August 11, 2018

Flash NanoPrecipitation for the Encapsulation of Hydrophobic and Hydrophilic Compounds in Polymeric Nanoparticles
10:12

Flash NanoPrecipitation for the Encapsulation of Hydrophobic and Hydrophilic Compounds in Polymeric Nanoparticles

Published on: January 7, 2019

  • Stability studies at 4°C and biological assays (gene silencing and expression) in relevant cell lines.

    • Main Results:

    • Consistent nanoparticle size (50-70 nm) and low PdI (<0.22) were achieved, irrespective of formulation parameters.
    • siRNA-loaded PBNs showed moderate size increase during storage; pDNA-loaded PBNs remained stable for 70 days.
    • Effective CDK4 gene silencing (~50%) and mCHERRY gene expression were confirmed in cellular assays.

    Conclusions:

    • WRAP5-based PBN formulation via microfluidics is robust and scalable.
    • These PBNs exhibit consistent physicochemical properties and potent biological activity.
    • WRAP5-PBNs present a viable and potentially less complex alternative to LNPs for nucleic acid delivery.