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Novel Acinetobacter baumannii Myovirus TaPaz Encoding Two Tailspike Depolymerases: Characterization and

Anastasia S Shchurova1,2, Mikhail M Shneider3, Nikolay P Arbatsky4

  • 1Moscow Institute of Physics and Technology (National Research University), Institutskiy per. 9, Dolgoprudny, 141700 Moscow Region, Russia.

Viruses
|June 2, 2021
PubMed
Summary

A novel bacteriophage, TaPaz, was isolated and studied. Its tailspike depolymerases show potential for targeting specific Acinetobacter baumannii capsular polysaccharide types, offering new avenues for combating this nosocomial pathogen.

Keywords:
Acinetobacter baumanniibacteriophagecapsular polysaccharidecapsular typeglycosidasetailspike depolymerase

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Area of Science:

  • Microbiology
  • Virology
  • Biochemistry

Background:

  • Acinetobacter baumannii is a major cause of hospital-acquired infections.
  • Bacteriophages targeting A. baumannii are crucial for controlling infections.
  • Capsular polysaccharides (CPSs) are key receptors for bacteriophages and influence bacterial virulence.

Purpose of the Study:

  • To characterize the novel myovirus TaPaz infecting A. baumannii.
  • To investigate the genomic organization and host interaction strategies of TaPaz.
  • To identify and evaluate the function of TaPaz's polysaccharide-degrading enzymes.

Main Methods:

  • Isolation and characterization of bacteriophage TaPaz.
  • Genome sequencing and analysis of TaPaz.
  • Purification and biochemical testing of recombinant tailspike depolymerases (TSDs).

Main Results:

  • TaPaz possesses a 93,703 bp double-stranded DNA genome with 178 open reading frames.
  • Two distinct tailspike depolymerase genes were identified within the TaPaz genome.
  • One recombinant TSD specifically cleaved the K47 capsular polysaccharide of A. baumannii via hydrolysis.

Conclusions:

  • The novel myovirus TaPaz offers insights into A. baumannii-phage interactions.
  • TaPaz encodes specific polysaccharide-degrading enzymes with potential therapeutic applications.
  • The characterized tailspike depolymerase demonstrates targeted activity against K47 A. baumannii strains.