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Protein Engineering of Electron Transfer Components from Electroactive Geobacter Bacteria
Tomás M Fernandes1, Leonor Morgado1, David L Turner2
1UCIBIO, Chemistry Department, NOVA School of Science and Technology, Universidade NOVA de Lisboa, Campus Caparica, 2829-516 Caparica, Portugal.
Antioxidants (Basel, Switzerland)
|June 2, 2021
Summary
Electrogenic bacteria like Geobacter transfer electrons externally, enabling microbial electrochemical technologies (METs) for bioremediation and bioenergy. Engineering their multiheme cytochromes (MCs) can enhance MET efficiency.
Area of Science:
- Microbiology
- Biochemistry
- Bioengineering
Background:
- Electrogenic microorganisms facilitate extracellular electron transfer (EET) and detoxify compounds.
- Microbial Electrochemical Technologies (METs) leverage these microbes for bioremediation and bioenergy.
- Geobacter bacteria are model organisms for studying EET, crucial for METs.
Purpose of the Study:
- To review the characteristics of electrogenic Geobacter bacteria and their role in METs.
- To highlight the importance of multiheme cytochromes (MCs) in EET.
- To discuss methodologies for characterizing MC redox networks and engineering Geobacter for improved MET performance.
Main Methods:
- Review of existing literature on electrogenic bacteria, METs, and MCs.
- Description of current methodologies for characterizing MC functional redox networks.
- Discussion of protein engineering strategies for MCs.
Main Results:
- Geobacter's EET capabilities are vital for MET applications.
- Multiheme cytochromes (MCs) are key mediators of EET.
- Characterization of MC redox networks is complex but essential for optimization.
Conclusions:
- Understanding MCs in Geobacter is critical for advancing METs.
- Rational protein engineering of MCs offers a pathway to enhance EET rates.
- Optimized Geobacter strains hold significant potential for bioremediation and bioenergy production.
Keywords:
Microbial Electrochemical Technologies (METs)Nuclear Magnetic Resonance (NMR)bioenergy productionbioremediationelectroactive microorganismsmultiheme cytochromesprotein engineeringredox characterizationMore Related Videos
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