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Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
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Portable Raman Spectrometer as a Screening Tool for Characterization of Iberian Dry-Cured Ham
Andrés Martín-Gómez1, Natalia Arroyo-Manzanares2, María García-Nicolás2
1Department of Analytical Chemistry, Institute of Fine Chemistry and Nanochemistry, Marie Curie Annex Building, Campus de Rabanales, 14071 Córdoba, Spain.
Foods (Basel, Switzerland)
|June 2, 2021
Summary
Raman spectroscopy can rapidly screen dry-cured Iberian ham, distinguishing breed and feeding. This method helps combat labelling fraud in the valuable food sector.
Area of Science:
- Food Science
- Analytical Chemistry
- Spectroscopy
Background:
- Dry-cured Iberian ham classification relies on breed and feeding, leading to price disparities.
- Labeling fraud in Iberian ham causes significant economic damage to the food industry.
Purpose of the Study:
- To explore Raman spectroscopy as a rapid, in situ screening tool for Iberian ham.
- To differentiate Iberian ham based on pig breed (purebred vs. crossbred) and feeding regime (acorn-fed vs. feed-fed).
Main Methods:
- Analyzed 110 dry-cured Iberian ham samples using Raman spectroscopy.
- Applied chemometric treatment to overcome fluorescence interference and extract spectral information.
- Conducted an interlaboratory study with 52 samples to validate the method's applicability.
Main Results:
- Achieved high validated classification rates: 83.3% for feeding regime and 86.7% for breed.
- The developed method demonstrated robustness in an interlaboratory setting, with validated rates exceeding 80%.
Conclusions:
- Raman spectroscopy, coupled with chemometrics, is a viable rapid screening tool for authenticating Iberian ham.
- This technique can effectively combat labeling fraud by accurately identifying breed and feeding practices.
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