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Visual Detection of Multiple Nucleic Acids in a Capillary Array

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Published on: November 15, 2017

Lab-on-a-foil devices with integrated retro-reflective structures for multiplexed DNA testing.

Pelin Toren¹, Andreas Proksch¹, Martin Smolka¹

¹Materials-Institute for Surface Technologies and Photonics, JOANNEUM RESEARCH Forschungsgesellschaft mbH, 8160 Weiz, Austria.

MRS Advances

|June 2, 2021

Summary

The COVID-19 pandemic highlighted the need for rapid, affordable virus testing. This study presents a novel roll-to-roll UV nanoimprint lithography method for mass-producing disposable in vitro diagnostic (IVD) biochips, enhancing DNA detection capabilities.

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Area of Science:

Biotechnology
Materials Science
Nanotechnology

Background:

The COVID-19 pandemic underscored the critical need for rapid, affordable, and scalable diagnostic testing systems.
Disposable biochips are essential for in vitro diagnostic (IVD) testing, requiring high-volume, rapid manufacturing.
Roll-to-roll (R2R) polymer structuring offers a viable solution for large-scale biochip production.

Purpose of the Study:

To demonstrate a novel R2R UV nanoimprint lithography (UV-NIL) process for manufacturing in vitro diagnostic (IVD) biochips.
To develop a Lab-on-a-Foil device for multiplexed DNA detection using capillary flow.
To enhance the performance of IVD biochips through the integration of microstructures for improved optical signal detection.

Main Methods:

Utilized R2R UV nanoimprint lithography (UV-NIL) for high-throughput fabrication of polymer-based biochip components.
Produced approximately 7500 biochip components per 100 meters of flexible polymer foil.
Integrated retro-reflective microstructures, fabricated via UV-NIL, into the biochip design to enhance optical signal detection.

Main Results:

Successfully generated a prototype foil-based IVD biochip for multiplexed DNA detection.
Demonstrated the capability to produce 7500 biochip components (e.g., capillary fluidic channels, optical structures) per 100m of polymer foil.
Showcased enhanced optical signal detection in a chemiluminescent-based DNA detection IVD device through integrated retro-reflective microstructures.

Conclusions:

R2R UV-NIL is a highly effective method for the mass production of advanced IVD biochips.
The developed Lab-on-a-Foil device with integrated microstructures significantly improves optical detection sensitivity for DNA analysis.
This technology holds promise for accelerating the availability of crucial diagnostic tools, particularly in response to global health crises.