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Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology
Published on: October 20, 2010
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Novel quinoline-based fluorescent bioimaging probe, KSNP117, for sentinel lymph node mapping.
Ahmed A Elbatrawy1, Da Sol Lee, Sang Bong Lee
1Center for Neuro-Medicine Brain Science Institute, Korea Institutes of Science and Technology Seoul, 02792, South Korea. gsnam@kist.re.kr.
Journal of Materials Chemistry. B
|June 2, 2021
Summary
A new quinoline-based fluorescent probe, KSNP117, shows promise for sensitive sentinel lymph node (SLN) detection. This biocompatible imaging agent demonstrates excellent stability and in vivo safety, aiding lymphatic mapping.
Area of Science:
- Biomedical Imaging
- Fluorescent Probes
- Molecular Imaging
Background:
- Accurate detection of sentinel lymph nodes (SLNs) is crucial for cancer staging and treatment planning.
- Current imaging agents often lack the required sensitivity, biocompatibility, and stability.
- Development of novel fluorescent probes is essential for advancing SLN detection.
Purpose of the Study:
- To design and evaluate a novel quinoline-based fluorescent probe, KSNP117, for sensitive and quantitative detection of SLNs.
- To assess the biocompatibility, serum stability, and photo-brightening effects of KSNP117.
- To investigate the in vivo performance of KSNP117 as a lymphatic tracer.
Main Methods:
- Synthesis and characterization of the quinoline-based fluorescent probe KSNP117.
- In vitro assessment of KSNP117's effects on ovary and immune cell proliferation.
- Evaluation of KSNP117's serum stability and photo-brightening properties.
- In vivo and ex vivo fluorescent imaging studies in nude mice to track SLN accumulation.
Main Results:
- KSNP117 demonstrated no adverse effects on cell proliferation and exhibited excellent serum stability.
- The probe showed photo-brightening effects, enhancing signal intensity.
- In vivo imaging confirmed rapid accumulation of KSNP117 in SLNs within 10 minutes post-injection.
- No in vivo toxicity was observed, consistent with ex vivo imaging findings.
Conclusions:
- KSNP117 is a novel, biocompatible, and highly sensitive fluorescent probe for SLN detection.
- The probe exhibits favorable stability and safety profiles for biomedical imaging.
- KSNP117 shows significant potential as a lymphatic tracer for accurate SLN mapping in clinical applications.
