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[Specific determination of free urinary cortisol using high performance liquid chromatography]
M C Patricot1, H Fraass, B Mathian
1Laboratoire d'Hormonologie, Centre hospitalier Lyon-Sud, Pierre-Bénite.
Annales De Biologie Clinique
|January 1, 1988
Summary
Measuring free urinary cortisol is challenging. This study compares purification methods for high-pressure liquid chromatography (HPLC) assays, proposing a routine protocol for accurate free cortisol measurement.
Area of Science:
- Clinical Chemistry
- Analytical Chemistry
- Biochemistry
Context:
- Accurate measurement of free urinary cortisol is crucial for diagnosing and monitoring conditions related to the hypothalamic-pituitary-adrenal axis.
- Existing methods for free cortisol assay are often complex or lack specificity.
- High-pressure liquid chromatography (HPLC) offers a promising analytical technique but necessitates effective sample pretreatment.
Purpose:
- To compare seven different urine purification protocols for free cortisol analysis using HPLC.
- To propose and validate a routine, efficient purification method for free urinary cortisol determination.
- To evaluate the performance of the proposed method in patients undergoing corticoid therapy.
Summary:
- Seven distinct urine purification protocols were evaluated for their suitability in quantifying free urinary cortisol via HPLC.
- A simplified, routine purification method was developed and optimized.
- The performance of this routine method was assessed, demonstrating its utility in clinical settings, particularly for patients on corticoid treatment.
Impact:
- Establishes a reliable and practical method for routine free urinary cortisol measurement.
- Facilitates more accurate clinical assessments and therapeutic monitoring in endocrinology.
- Improves the diagnostic capabilities for Cushing's syndrome and monitoring exogenous steroid use.