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MicroRNA miR-92a-3p regulates breast cancer cell proliferation and metastasis via regulating B-cell translocation

Huang Jinghua1, Zhou Qinghua2, Chen Chenchen3

  • 1Department of Radiation Oncology, Affiliated Hospital of Yanbian University, Yanbian, Jilin, China.

Bioengineered
|June 4, 2021
PubMed

Insights

MicroRNA miR-92a-3p promotes breast cancer (BC) cell growth and spread. Inhibiting miR-92a-3p may offer a new therapeutic strategy for breast cancer by targeting BTG2.

Area of Science:

  • Molecular Biology
  • Oncology

Background:

  • MicroRNA (miRNA) dysregulation is implicated in tumorigenesis.
  • Aberrant miR-92a-3p expression is linked to cancer development and progression.
  • Understanding miR-92a-3p's role in breast cancer (BC) is crucial.

Purpose of the Study:

  • To investigate the function and regulatory mechanism of miR-92a-3p in breast cancer.
  • To determine the relationship between miR-92a-3p expression and clinical parameters in BC patients.

Main Methods:

  • Quantitative real-time PCR to assess miR-92a-3p expression in BC tissues and cells.
  • Cell proliferation and metastasis assays following transfection with miR-92a-3p mimics or inhibitors.
  • Western blotting and luciferase reporter assays to validate BTG2 as a direct target of miR-92a-3p.

Main Results:

  • miR-92a-3p expression was significantly upregulated in BC tissues and cells.
  • High miR-92a-3p expression correlated with advanced TNM stage and larger tumor size.
  • Overexpression of miR-92a-3p enhanced BC cell proliferation and metastasis, while inhibition suppressed these processes.
  • BTG2 was identified as a direct downstream target repressed by miR-92a-3p.

Conclusions:

  • miR-92a-3p acts as an oncogenic miRNA in breast cancer.
  • miR-92a-3p promotes BC cell proliferation and metastasis by downregulating BTG2.
  • Targeting miR-92a-3p may represent a potential therapeutic strategy for breast cancer.
Keywords:
BTG2Breast cancermiR-92a-3p

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