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Phase separation during blood spreading.

Houssine Benabdelhalim1, David Brutin2

  • 1Aix Marseille Univ, CNRS, IUSTI, Marseille, France. houssine.benabdelhalim@gmail.com.

Scientific Reports
|June 4, 2021
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Summary
This summary is machine-generated.

Higher humidity increases blood pool area and serum spreading on surfaces. This bloodstain pattern analysis is crucial for crime scene investigation, especially on varnished wood. Understanding blood behavior aids forensic science.

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Area of Science:

  • Forensic Science
  • Biophysics
  • Material Science

Background:

  • Bloodstain pattern analysis is vital in forensic investigations.
  • Understanding blood spreading dynamics on various substrates is essential for interpreting crime scenes.
  • Environmental factors significantly influence blood pool morphology and phase separation.

Purpose of the Study:

  • To investigate the effect of relative humidity and temperature on blood pool spreading.
  • To analyze the phenomenon of serum phase separation from the main blood pool.
  • To determine the influence of substrate type (varnished vs. unvarnished wood) on blood spreading.

Main Methods:

  • Controlled experiments were conducted using constant masses of whole blood.
  • Blood pools were created on varnished and unvarnished wooden substrates.
  • Experiments were performed at varying temperatures (20°C, 25°C, 30°C) and relative humidity (20%-90%).

Main Results:

  • Increased relative humidity led to a larger final blood pool area.
  • Serum phase separation occurred at relative humidity levels above 50% across all tested temperatures.
  • Phase separation was more pronounced on varnished substrates, without altering drying morphology.

Conclusions:

  • Relative humidity is a key factor influencing blood pool area and serum spreading.
  • The competition between blood coagulation and evaporation explains the observed phase separation.
  • Findings provide valuable insights for forensic investigators analyzing bloodstains at crime scenes.