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Updated: Nov 3, 2025

Light-Controlled Fermentations for Microbial Chemical and Protein Production
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[Metabolic regulation in constructing microbial cell factories].

Yang Liu1, Qingxuan Mu1, Ya'nan Shi1

  • 1CAS Key Laboratory of Microbial Physiological & Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|June 4, 2021
PubMed
Summary

Metabolic regulation is key for microbial cell factories. Advances in synthetic biology enable precise, dynamic control of gene expression for improved metabolic engineering over the last 30 years.

Keywords:
metabolic engineeringmicrobial cell factoriesregulation of gene expressionsynthetic biology

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Area of Science:

  • Synthetic biology
  • Metabolic engineering
  • Systems biology

Background:

  • Metabolic regulation is crucial for designing efficient microbial cell factories.
  • Synthetic biology advancements have enhanced the ability to mine and design regulatory elements.
  • Research has shifted from static single-gene regulation to dynamic, systems-level control.

Purpose of the Study:

  • To review the technological advancements in metabolic regulation over the past three decades.
  • To highlight the evolution of metabolic regulation strategies.
  • To provide an overview of current capabilities in microbial metabolic engineering.

Main Methods:

  • Review of scientific literature on metabolic regulation technologies.
  • Analysis of the progression of synthetic biology tools for metabolic control.
  • Synthesis of information on systems-level metabolic regulation.

Main Results:

  • Significant improvements in regulatory element design and mining.
  • Development of sophisticated tools for modifying cellular metabolic networks.
  • Transition towards intelligent and precise dynamic regulation strategies.

Conclusions:

  • Metabolic regulation technologies have rapidly advanced, driven by synthetic biology.
  • Dynamic, systems-level regulation offers greater precision for metabolic engineering.
  • The field has evolved significantly, enabling more efficient microbial cell factories.