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Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
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Engineering of a LysG-derived arginine-specific biosensor for high-throughput screening of arginine overproducers in Corynebacterium glutamicum.

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[Recent advances in developing enabling technologies for Corynebacterium glutamicum metabolic engineering].

Yu Wang1,2, Ping Zheng1,2, Jibin Sun1,2

  • 1Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin Institute of Industrial Biotechnology, Tianjin 300308, China.

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|June 4, 2021
PubMed
Summary

Corynebacterium glutamicum is a key microbe for amino acid production. Recent advances in metabolic engineering technologies, including CRISPR, enhance its use as a microbial factory for biotechnology.

Keywords:
Corynebacterium glutamicumadaptive laboratory evolutionbiosensorexpression regulationgenome editingmetabolic engineering

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Area of Science:

  • Industrial Biotechnology
  • Microbial Engineering
  • Synthetic Biology

Background:

  • Corynebacterium glutamicum is a vital industrial microorganism, producing over 6 million tons of amino acids annually.
  • Metabolic engineering of C. glutamicum is crucial for optimizing amino acid biosynthesis and expanding its industrial applications.

Purpose of the Study:

  • To review recent technological advancements in C. glutamicum metabolic engineering.
  • To highlight the establishment and applications of cutting-edge tools for microbial cell factory development.

Main Methods:

  • CRISPR-based genome editing for precise genetic modifications.
  • Gene expression regulation techniques for controlling metabolic pathways.
  • Adaptive laboratory evolution for strain improvement.
  • Development and application of biosensors for monitoring and optimization.

Main Results:

  • Accelerated construction and optimization of microbial cell factories.
  • Expanded substrate utilization and product portfolios.
  • Facilitated fundamental research on C. glutamicum.

Conclusions:

  • C. glutamicum is an ideal microbial chassis due to recent technological progress.
  • These enabling technologies significantly advance C. glutamicum's role in industrial biotechnology and amino acid production.