Related Experiment Video
Updated: Nov 3, 2025

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
Published on: October 2, 2021
Extended measuring depth dual-wavelength Fourier domain optical coherence tomography.
Haroun Al-Mohamedi1, Ismael Kelly-Pérez1,2, Theo Oltrup3
1Sektion für Experimentelle Ophthalmochirurgie, Universitätsklinikum Tübingen, Tübingen, Germany.
This study introduces an enhanced dual band spectral domain optical coherence tomography (SD-OCT) system for more accurate optical A-scan biometry measurements. The improved technique increases measurement depth and precision, validated with a model eye.
Area of Science:
- Biomedical Optics
- Ophthalmology
- Medical Imaging
Background:
- Accurate optical A-scan biometry is crucial for refractive error assessment.
- Existing spectral domain optical coherence tomography (SD-OCT) techniques face limitations in depth and accuracy.
Purpose of the Study:
- To present an enhanced wide range dual band spectral domain optical coherence tomography (SD-OCT) technique.
- To improve the depth and accuracy of optical A-scan biometry measurements.
Main Methods:
- Utilized a Michelson interferometer with two wide-spectrum Superluminescent Diodes (SLDs).
- Employed a long-pass filter (900 nm) and a reflective diffraction grating (1,800 lines/mm).
- Captured the spectrally decomposed light using two CCD line sensors.
Main Results:
- Demonstrated an enhanced wide range dual band SD-OCT system.
- Achieved increased depth and accuracy in optical A-scan biometry measurements.
- Validated system capabilities using a self-made human model eye.
Conclusions:
- The presented enhanced dual band SD-OCT technique offers improved performance for optical biometry.
- The system shows potential for more precise ophthalmic measurements.
Related Concept Videos
Imaging Biological Samples with Optical Microscopy
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Phase Contrast and Differential Interference Contrast Microscopy
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

