Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Clinical Applications of Epidermal Stem Cells01:19

Clinical Applications of Epidermal Stem Cells

3.0K
Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...
3.0K
Burn Injuries01:22

Burn Injuries

3.2K
Burn injuries occur when the skin and underlying tissues are damaged due to exposure to heat, electricity, chemicals, radiation, or friction. They can vary in severity, from minor superficial burns to severe deep burns that can be life-threatening.
The damage results in the death of skin cells, which can lead to a massive loss of fluid. Dehydration, electrolyte imbalance, and renal and circulatory failure follow, which can be fatal. Burn patients are treated with intravenous fluids to offset...
3.2K
Phases of Wound Repair01:28

Phases of Wound Repair

7.0K
Following injury, the integrity of the injured tissues must be reestablished. For example, in skin tissue, wound repair involves coordination among resident skin cells, blood mononuclear cells, extracellular matrix, growth factors, and cytokines to complete the healing cascade.
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
7.0K
Overview of Regeneration and Repair01:19

Overview of Regeneration and Repair

4.4K
Regeneration and repair processes are critical in healing damages caused by injury, disease, and aging. In regeneration, the damaged tissue is entirely replaced with new growth that restores the original architecture and function. In contrast, tissue repair usually results in a fixed tissue architecture involving scar formation. Scars generally do not reestablish tissue function and may also exhibit structural abnormalities at the injury site.
Regeneration
All animals have varying degrees of...
4.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Safety of a gastropexy device in infants and young children in percutaneous endoscopic gastrostomy tube placement.

Scientific reports·2025
Same author

Stakeholder and patient experience with virtual reality in burn treatment - a study of the Cold River application in a clinical setting.

Acta chirurgiae plasticae·2025
Same author

Corrigendum for: Early Uterine Transplant Graft Loss Due to Thrombosis: Single-Center Experience With Causes, Prevention, Diagnosis, and Treatment.

Physiological research·2024
Same author

Comparison of "IN-REC-SUR-E" and LISA in preterm neonates with respiratory distress syndrome: a randomized controlled trial (IN-REC-LISA trial).

Trials·2024
Same author

Optimizing ischemic preconditioning for esophagogastric anastomosis: A standardized rat model to evaluate histological changes.

Rozhledy v chirurgii : mesicnik Ceskoslovenske chirurgicke spolecnosti·2024
Same author

Early Uterine Transplant Graft Loss Due to Thrombosis: Single-Center Experience With Causes, Prevention, Diagnosis, and Treatment.

Physiological research·2023
Same journal

MiR-335-3p Alleviates Adipogenesis and Inflammation in Bone Marrow-Derived Mesenchymal Stem Cells in Osteoporotic Fractures.

Folia biologica·2026
Same journal

Research on the Diagnostic Potential of miR-383-5p in Rheumatoid Arthritis.

Folia biologica·2026
Same journal

The Predictive Value of NLRP3-Mediated Inflammatory Factor Production for Abnormal Liver Function in Pregnant Women with Hepatitis B.

Folia biologica·2026
Same journal

Mechanism Study and Analysis of Correlation between miR-409-3p and Post-Stroke Cognitive Impairment.

Folia biologica·2026
Same journal

Correlation between circ_0092576 and Arterial Stenosis Severity and Inflammatory Status in Patients with Coronary Heart Disease and Its Clinical Value.

Folia biologica·2026
Same journal

Intravitreal Application of Mesenchymal Stem Cell By-Products Does Not Ameliorate Experimental Autoimmune Uveitis.

Folia biologica·2026
See all related articles

Related Experiment Video

Updated: Nov 3, 2025

A Murine Model of a Burn Wound Reconstructed with an Allogeneic Skin Graft
12:18

A Murine Model of a Burn Wound Reconstructed with an Allogeneic Skin Graft

Published on: August 8, 2020

10.4K

Early Changes during Skin Repair Using Tissue-Engineered Dermal Template in a Full-Thickness Burn.

P Gal1,2,3, L Frohlichova4, M Coma2,5

  • 1Prague Burn Centre, Third Faculty of Medicine, Charles University and Faculty Hospital Královské Vinohrady, Prague, Czech Republic.

Folia Biologica
|June 5, 2021
PubMed
Summary
This summary is machine-generated.

Integra artificial skin aids healing in severe burns. This tissue-engineered dermal template supports immune cell infiltration and T-cell presence, promoting safe wound closure.

More Related Videos

Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding
08:35

Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding

Published on: February 26, 2015

19.5K
Chessboard-like Burn Wound Healing Model of Mice Based on Digital Heating Device
04:04

Chessboard-like Burn Wound Healing Model of Mice Based on Digital Heating Device

Published on: December 27, 2024

1.0K

Related Experiment Videos

Last Updated: Nov 3, 2025

A Murine Model of a Burn Wound Reconstructed with an Allogeneic Skin Graft
12:18

A Murine Model of a Burn Wound Reconstructed with an Allogeneic Skin Graft

Published on: August 8, 2020

10.4K
Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding
08:35

Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding

Published on: February 26, 2015

19.5K
Chessboard-like Burn Wound Healing Model of Mice Based on Digital Heating Device
04:04

Chessboard-like Burn Wound Healing Model of Mice Based on Digital Heating Device

Published on: December 27, 2024

1.0K

Area of Science:

  • Regenerative Medicine
  • Biomaterials Science
  • Immunohistology

Background:

  • Rapid wound closure is critical for extensively burned patients.
  • Integra® is a widely used artificial skin composed of bovine collagen and chondroitin sulfate with a silicone layer.

Purpose of the Study:

  • To evaluate the (immuno)histological response to Integra® in a severely burned patient.
  • To assess immune cell infiltration and T-cell subpopulation presence in the artificial dermis.

Main Methods:

  • A severely burned patient was treated with Integra® and a split-thickness skin graft.
  • Immunohistochemical analysis was performed on the artificial dermis at various time points.
  • Tissue immunophenotyping identified immune cell markers (CD3+, CD4+, CD8+, CD56+).

Main Results:

  • Immune cell infiltration peaked around day 10 post-treatment.
  • CD3+ T-cells increased throughout the study.
  • CD4+ and CD8+ T-cells were present on day 40, while NK cells (CD56+) showed weak/no infiltration.

Conclusions:

  • The bi-layer Integra® artificial skin is a feasible and safe option for treating severe burns.
  • It facilitates the formation of non-irritating dermal substitutes.
  • The study highlights the role of immune responses in Integra®-based wound healing.