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Published on: January 7, 2019
Modification of Metal-Organic Framework Nanoparticles Using Dental Pulp Mesenchymal Stem Cell Membranes to Target
Dabo Zhou1, Yixin Chen2, Wenhuan Bu1
1School and Hospital of Stomatology, China Medical University, 117 Nanjing North Street, Shenyang 110002, China.
Abstract:
Engineering a targetable nanoparticle to tumor cell is a challenge issue for clinical application. Our results demonstrated that the chemokine CXCL8 secreted by oral squamous cell carcinoma (OSCC) could act as a chemoattractant to attract dental pulp mesenchymal stem cell (DPSC), which expressed the CXCL8 binding receptor, CXCR2, to the OSCC. Therefore, to create OSCC targetable nanoparticles, we used DPSC membranes to modify nanoparticles of metal-organic framework nanoparticles (MOFs) resulting in a novel MOF@DPSCM nanoparticle. Interestingly, results from in vitro and in vivo experiments illustrated that MOF@DPSCM possessed specificity for the OSCC, and the MOF@DPSCM carried DOX (doxorubicin), MOF-DOX@DPSCM could induce CAL27 cell death in vitro and block CAL27 tumor growth in vivo. Our data suggest that this novel MOF-DOX@DPSCM nanoparticle is a potential targetable drug delivery system for the OSCC in the future clinical application.
Insights
Researchers engineered a novel nanoparticle using dental pulp mesenchymal stem cell membranes to target oral squamous cell carcinoma (OSCC). This targeted nanoparticle loaded with doxorubicin effectively killed cancer cells and inhibited tumor growth in vivo.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Oncology
Background:
- Targeting cancer cells with nanoparticles remains a significant challenge in clinical applications.
- Oral squamous cell carcinoma (OSCC) secretes chemokine CXCL8, attracting dental pulp stem cells (DPSCs) expressing the CXCL8 receptor, CXCR2.
Purpose of the Study:
- To develop a novel nanoparticle system targeting OSCC by utilizing the inherent cell-targeting properties of DPSCs.
- To evaluate the efficacy of the DPSC-membrane-coated metal-organic framework nanoparticles (MOFs) loaded with doxorubicin (DOX) for OSCC treatment.
Main Methods:
- Modification of metal-organic framework nanoparticles (MOFs) with dental pulp mesenchymal stem cell (DPSC) membranes to create MOF@DPSCM nanoparticles.
- In vitro and in vivo experiments to assess the targeting specificity and anti-cancer efficacy of DOX-loaded MOF@DPSCM (MOF-DOX@DPSCM).
Main Results:
- MOF@DPSCM nanoparticles demonstrated specific targeting of OSCC cells.
- MOF-DOX@DPSCM effectively induced cancer cell death in vitro.
- MOF-DOX@DPSCM significantly inhibited tumor growth in vivo models.
Conclusions:
- The novel MOF-DOX@DPSCM nanoparticle system exhibits promising OSCC-targeting capabilities.
- This DPSC-membrane-coated nanoparticle represents a potential targeted drug delivery strategy for oral squamous cell carcinoma.
