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Mass Spectrometry: Isotope Effect

Mass Spectrometry: Isotope Effect

Most elements exist in nature as a mixture of isotopes. The isotopes differ in weight due to their respective number of neutrons. The molecular weight of a molecule is different depending on the specific isotope of its elements involved. As a result, the mass spectrum of the molecule exhibits peaks from the same fragment at multiple positions. The positions of these mass signals depend on the difference between the molecular mass. Furthermore, the intensity of these signals is dependent on the...

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Updated: Nov 3, 2025

Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling

Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling

Published on: July 1, 2014

Chemical isotope labeling for quantitative proteomics.

Xiaobo Tian¹, Hjalmar P Permentier¹, Rainer Bischoff¹

¹Department of Analytical Biochemistry and Interfaculty Mass Spectrometry Center, Groningen Research Institute of Pharmacy, University of Groningen, Groningen, The Netherlands.

Mass Spectrometry Reviews

|June 6, 2021

Summary

Multiplex isotope labeling enhances accuracy and efficiency in mass spectrometry-based proteome quantification. This review covers chemical isotope labeling methods for quantitative proteomics, aiding technique selection.

Keywords:

fragment ion isobaric labeling quantitative proteomics stable isotope labeling tandem mass spectrometry

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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

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Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling

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Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT

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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Area of Science:

Biochemistry
Analytical Chemistry
Proteomics

Background:

Liquid chromatography and mass spectrometry advancements drive proteome quantification.
Multiplex isotope labeling improves accuracy, precision, and throughput in quantitative proteomics.
Isotope labeling methods are crucial for analyzing complex biological samples.

Purpose of the Study:

To provide an overview of chemical isotope labeling approaches for quantitative proteomics.
To discuss the principles, benefits, and limitations of various isotope labeling methods.
To offer guidance for selecting appropriate quantitative proteomics techniques.

Main Methods:

Review of chemical isotope labeling strategies.
Analysis of MS1-based and MS2-based quantification.
Discussion of data-dependent acquisition (DDA) and data-independent acquisition (DIA) modes.

Main Results:

Chemical isotope labeling offers significant improvements in quantitative proteomics.
Different labeling strategies present unique advantages and disadvantages.
Multiplexing enhances the power of isotope labeling for complex analyses.

Conclusions:

Isotope labeling is a vital tool for accurate proteome quantification.
Understanding method principles is key to selecting the best approach.
Future directions include optimizing labeling for data-independent acquisition.