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Related Experiment Video

Updated: Nov 2, 2025

Following the Dynamics of Structural Variants in Experimentally Evolved Populations

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Published on: February 3, 2023

Dynamic changes in DNA populations revealed by split-combine selection.

Olga Plückthun¹, Julia Siegl¹, Laura Lledo Bryant¹

¹Chemical Biology and Chemical Genetics, Life and Medical Sciences (LIMES) Institute, University of Bonn Gerhard-Domagk-Str. 1 53121 Bonn Germany gmayer@uni-bonn.de.

Chemical Science

|June 7, 2021

Summary

Chemically modified aptamers, or clickmers, offer new ways to find biomolecule binders for therapies and diagnostics. A novel split-combine selection strategy tracks DNA changes, revealing which clickmers best adapt to specific conditions.

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Area of Science:

Biochemistry
Molecular Biology
Chemical Biology

Background:

Clickmers are chemically modified aptamers, a novel class of reagents for biomolecule binder development.
These binders have significant potential in therapeutic and diagnostic applications.
Current screening methods lack the ability to simultaneously enrich diverse chemical modifications.

Purpose of the Study:

To develop a novel split-combine selection strategy for screening clickmers with diverse chemical modifications.
To enable simultaneous enrichment of clickmers with varying chemical entities.
To provide molecular-level insights into the selection process.

Main Methods:

Development of a split-combine selection strategy.
Simultaneous enrichment of clickmers with different chemical modifications.
Tracking of dynamic DNA population changes at the individual sequence level.
Off-rate guided enrichment to observe sequence adaptation.

Main Results:

The split-combine strategy allows simultaneous enrichment of clickmers with diverse modifications.
Dynamic changes in DNA populations are traceable at the individual sequence level.
The process identifies sequences best adapted to specific selection conditions, observable through survival.
Unprecedented molecular insight into the selection process is achieved.

Conclusions:

The developed split-combine selection strategy offers a novel approach for screening clickmers.
This method provides deep molecular insights into the selection dynamics.
The strategy facilitates the observation of sequence adaptation and survival under selection pressure.
Future development of more sophisticated selection procedures is enabled by this approach.