Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

8.7K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ligation-Driven Electrochemical Magneto-Genoassay Platform Based on PNA Probes for the Multiple Detection of Soy and Mustard DNA in Wheat Flour.

Biosensors·2026
Same author

From alkylating to shape-shifting G-quadruplex ligands: the RHAU peptide story.

Nucleic acids research·2026
Same author

Versatile introduction of multifunctional Michael-acceptor moieties on amino-oligonucleotides for bioconjugation purposes.

Communications chemistry·2026
Same author

Higher-Level Structural Classification of Pseudomonas Cyclic Lipopeptides through Their Bioactive Conformation.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

Chemically modified CRISPR-Cas9 enables targeting of individual G-quadruplex and i-motif structures, revealing ligand-dependent transcriptional perturbation.

Nature communications·2025
Same author

From loops to caps: discriminating peptide binding to distinct G-quadruplex tetrads using 5-furyl-2'-deoxyuridine fluorescent probes.

RSC chemical biology·2025

Related Experiment Video

Updated: Nov 2, 2025

Visualization of Surface-tethered Large DNA Molecules with a Fluorescent Protein DNA Binding Peptide
08:51

Visualization of Surface-tethered Large DNA Molecules with a Fluorescent Protein DNA Binding Peptide

Published on: June 23, 2016

11.0K

Visible-light triggered templated ligation on surface using furan-modified PNAs.

Alex Manicardi1, Enrico Cadoni1, Annemieke Madder1

  • 1Organic and Biomimetic Chemistry Research Group, Department of Organic and Macromolecular Chemistry, Ghent University Krijgslaan 281-S4 9000 Gent Belgium Alex.Manicardi@Ugent.be Annemieke.Madder@Ugent.be.

Chemical Science
|June 7, 2021
PubMed
Summary

This study introduces a novel surface-based reaction for detecting nucleic acid targets. Visible light triggers templated ligation using peptide nucleic acids (PNAs), enabling sensitive detection in a high-throughput format.

More Related Videos

Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography
11:05

Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography

Published on: October 25, 2018

7.7K
Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy
10:59

Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy

Published on: October 18, 2024

917

Related Experiment Videos

Last Updated: Nov 2, 2025

Visualization of Surface-tethered Large DNA Molecules with a Fluorescent Protein DNA Binding Peptide
08:51

Visualization of Surface-tethered Large DNA Molecules with a Fluorescent Protein DNA Binding Peptide

Published on: June 23, 2016

11.0K
Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography
11:05

Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography

Published on: October 25, 2018

7.7K
Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy
10:59

Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy

Published on: October 18, 2024

917

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Nanotechnology

Background:

  • Oligonucleotide-templated reactions are crucial for biosensors and DNA-based nanotechnology.
  • Translating solution-based template chemistry to surfaces for high-throughput systems remains challenging.

Purpose of the Study:

  • To demonstrate the first visible light-triggered templated ligation reaction on a surface.
  • To develop a sensitive method for nucleic acid detection using peptide nucleic acids (PNAs).

Main Methods:

  • Utilized furan-modified PNAs with a photosensitizer and nucleophilic moiety.
  • Employed visible white light irradiation to activate furan via localized singlet oxygen production.
  • Leveraged target-induced proximity for template-directed ligation.

Main Results:

  • Achieved successful surface-based, light-triggered PNA ligation.
  • Demonstrated sensitive detection of DNA and RNA targets using a 22mer template.
  • Developed a method unaffected by probe dilution or reducing conditions.

Conclusions:

  • This work presents a new surface chemistry for oligonucleotide-templated reactions.
  • The method offers a sensitive and selective approach for nucleic acid detection in a 96-well plate format.
  • Opens new avenues for DNA-based materials and high-throughput biosensing applications.