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Related Experiment Video

Updated: Nov 2, 2025

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform
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Microfluidic and Nanofluidic Intracellular Delivery.

Jeongsoo Hur1, Aram J Chung2

  • 1School of Biomedical Engineering, Korea University, Seoul, 02841, Republic of Korea.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|June 7, 2021
PubMed
Summary

Microfluidic and nanofluidic technologies offer advanced methods for intracellular delivery of biomolecules, overcoming limitations of traditional techniques. These innovative approaches enhance cell engineering for applications like gene editing and cancer immunotherapy.

Keywords:
cell transfectiongene deliveryintracellular deliverymicrofluidicsnanofluidics

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Bioengineering

Background:

  • Cellular reprogramming involves introducing biomolecules like DNA, RNA, proteins, or nanomaterials into cells.
  • Traditional intracellular delivery methods (viral vectors, electroporation) often compromise cell viability and function.
  • Gene editing (e.g., CRISPR-Cas9) and immunotherapy (e.g., CAR-T cells) rely on efficient intracellular delivery.

Purpose of the Study:

  • To review recent advancements in microfluidic and nanofluidic strategies for intracellular delivery.
  • To discuss the potential and challenges of these technologies for clinical applications.
  • To outline future directions for developing next-generation intracellular delivery platforms.

Main Methods:

  • Review of current literature on microfluidic and nanofluidic intracellular delivery techniques.
  • Analysis of emerging trends and their implications for cell engineering.
  • Discussion of clinical translation considerations.

Main Results:

  • Microfluidic and nanofluidic approaches show significant potential for high-efficiency intracellular delivery.
  • These methods offer improved preservation of cell viability, phenotype, and function compared to traditional tools.
  • Emerging solutions address key challenges in delivering diverse biomolecules into cells.

Conclusions:

  • Microfluidics and nanofluidics represent promising next-generation platforms for intracellular delivery.
  • Further development is needed to optimize these technologies for widespread clinical application.
  • These advanced techniques are poised to revolutionize cell engineering and therapeutic development.