Home Detection Technique for Na⁺ and K⁺ in Urine Using a Self-Calibrated all-Solid-State Ion-Selective Electrode...

An all-solid-state ion-selective electrode (ASS-ISE) array that is portable and easily miniaturized can meet the needs of home sensing devices for long-term health monitoring. However, their stability and accuracy are affected by the multistep modification required for ASS-ISE manufacturing and the complex background signal of real samples. In this study, a four-channel ISE array with the integration of a calibration channel has been developed based on polystyrene-Au (PS-Au) ion-sensing nanocomposites (PS-Au ISE array) for the home detection of Na⁺ and K⁺. The nanocomposites combine target recognition function and ion-electron transduction function and could be modified on the channel surface by direct drop-casting, thus simplifying the preparation process and then improving the stability. Meanwhile, the integrated calibration channel could automatically deduct complex background signals in real sample analysis and thus improve the accuracy. As a result, the proposed self-calibrated PS-Au ISE array showed a near Nernstian behavior for Na⁺ and K⁺ in the range of 1 × 10^-2 M-1 × 10^-4 M, and the detection limits were 6.8 × 10^-5 M and 5.5 × 10^-5 M in artificial urine. The linear equations can be obtained according to the slopes and intercepts of Na⁺ and K⁺, and thus, the concentration of the target ions can be directly read out by combining this PS-Au ISE array with the smart electronic device. Furthermore, the detection results of Na⁺ and K⁺ in human urine agreed well with those obtained by ICP-AES, suggesting that this proposed self-calibrated PS-Au ISE array is very suitable for home smart sensing devices, facilitating the health monitoring.