Exploring DNA in biochemistry lab courses: DNA barcoding and phylogenetic analysis.

Area of Science:

• Molecular Biology
• Bioinformatics
• Genetics

Background:

• DNA analysis is crucial for species identification, evolutionary studies, and individual identification.
• Existing methods can be complex for introductory laboratory settings.

Purpose of the Study:

• To present a practical, hands-on DNA barcoding method for university laboratory courses.
• To integrate DNA isolation, purification, PCR, sequencing, and phylogeny analysis into a cohesive learning experience.
• To provide a pipeline for identifying unknown samples, mirroring research practices.

Main Methods:

• Utilized DNA from commercially available mushrooms for laboratory exercises.
• Developed a three-part laboratory assignment sequence.
• Incorporated DNA isolation, purification, polymerase chain reaction (PCR), DNA sequencing, and phylogenetic analysis.
• Designed for a university teaching laboratory setting, with one assignment adaptable for remote learning.

Main Results:

• Successfully created a comprehensive DNA barcoding pipeline for educational purposes.
• Demonstrated the application of standard molecular techniques in a practical, experimental context.
• Enabled students to perform experimental design, sample preparation, and utilize molecular instruments.

Conclusions:

• The presented DNA barcoding method effectively teaches core molecular biology principles and techniques.
• The curriculum provides a valuable, hands-on approach to species identification and phylogenetic analysis in an academic setting.
• The modular design allows for flexible integration into various course structures, including remote learning environments.