Mutagenicity Evaluation of Nanoparticles by the Ames Assay

The Ames assay is a classic and robust method for identifying and evaluating chemical mutagens that reverse the mutations of Salmonella typhimurium and/or Escherichia coli bacteria strains with amino acid synthesis defects. It is also called the bacterial reverse mutation assay. Ames assay has been widely used for detecting genetic toxicity of many chemicals and gained increased applications in risk assessment of emerging environmental pollutants such as nanomaterials. In this chapter, we presented a detailed step-by-step method using the Ames assay to detect potential mutagenicity of metal oxide nanoparticles. The strategy to use the liver S9 fraction for bioactivation and a preincubation procedure is recommended. This method is easy to use to test genetic toxicity of other environmental contaminants and new chemicals.