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Transmission electron microscopy of small numbers of sorted cells
R J Sebring1, N F Johnson, W D Spall
1Life Sciences Division, Los Alamos National Laboratory, New Mexico 87545.
Cytometry
|January 1, 1988
Abstract:
A new method that allows the transmission electron microscopic examination of as few as 1 x 10(4) cells obtained by flow cytometric sorting is described. The approach involves "sandwiching" fixed cells in an agarose case by a microcentrifugation system consisting of small-diameter cell-centrifugation tubes and subsequent processing of the cells by conventional techniques. The advantages offered by this method are discussed.