Three-Dimensionally Printed Skin Substitute Using Human Dermal Fibroblasts and Human Epidermal Keratinocytes

Area of Science:

• Biotechnology
• Tissue Engineering
• Regenerative Medicine

Background:

• Wound healing is a complex biological process crucial for restoring tissue integrity.
• Large wounds often necessitate skin grafting, highlighting the need for alternative therapeutic strategies.
• 3D bioprinting presents a promising avenue for fabricating functional tissue substitutes.

Purpose of the Study:

• To investigate the feasibility of 3D bioprinting viable skin substitutes.
• To determine if human keratinocytes and dermal fibroblasts can be successfully printed into a skin-like structure.
• To explore novel strategies for enhancing wound healing through tissue engineering.

Main Methods:

• Adult human dermal fibroblasts (HDFa) and epidermal keratinocytes (HEKa) were cultured.
• Cells were suspended in a hydrogel and printed using a 3D bioprinter.
• Printed constructs were analyzed for cell viability and histological appearance via hematoxylin and eosin staining.

Main Results:

• Human epidermal keratinocytes (HEKa) and dermal fibroblasts (HDFa) were successfully encapsulated and printed within a hydrogel scaffold.
• The 3D-printed constructs exhibited a histological appearance resembling native skin.
• Cell viability was maintained throughout the bioprinting process.

Conclusions:

• Human epidermal keratinocytes (HEKa) and dermal fibroblasts (HDFa) can be successfully bioprinted into 3D scaffolds.
• The bioprinted cells survive and proliferate within the hydrogel matrix.
• This technique holds potential for generating viable skin tissue for wound healing and reconstructive surgery.