Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Crystal Chemistry of NaRE(CO<sub>3</sub>)<sub>2</sub> across Lanthanide Series, Y, and Sc.

Inorganic chemistry·2026

Same author

Fluidic Lipid-Bilayer-Enhanced Iontronic Nanopore: Machine-Learning-Driven Ultrasensitive MicroRNA Detection in Cancer Diagnostics.

ACS sensors·2026

Same author

Recent Advances in Direct Single Oxygen Atom Insertion Into Organic Frameworks.

Chemistry, an Asian journal·2026

Same author

In Situ Translation of Terminal Glycans into Covalent Readouts in Complex Biological Systems.

Analytical chemistry·2026

Same author

Supramolecular Organic Framework-Enabled AIE-Enhanced Electrochemiluminescence: Dual-Readout Ultrasensitive Determination of Microcystin-LR in Environmental Waters.

Analytical chemistry·2026

Same author

Electron-Ion Coupling for Nanostructured Photocathode in Ion-Assisted Photoelectrochemical Microfluidic Biosensing Platform.

Analytical chemistry·2026

Search research articles

Related Experiment Video

Updated: Nov 2, 2025

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

Split-Type Electrochemical Immunoassay System Triggering Ascorbic Acid-Mediated Signal Magnification Based on a

Liu Qu¹, Xiang Ren¹, Dawei Fan¹

¹Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022 Shandong, China.

ACS Applied Materials & Interfaces

|June 8, 2021

Summary

A novel electrochemical immunosensor uses a controlled-release strategy for sensitive neuron-specific enolase (NSE) detection. This biosensor integrates ascorbic acid (AA) release with a gold nanoparticle/porous bismuth vanadate platform for signal amplification.

Keywords:

SBGCdS-AA controlled-release strategy neuron-specific enolase split-type EC immunosensor synergetic catalysis oxidation

More Related Videos

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Published on: November 13, 2017

Related Experiment Videos

Last Updated: Nov 2, 2025

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

Published on: September 19, 2017

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Published on: February 23, 2020

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Published on: November 13, 2017

Area of Science:

Electrochemistry
Biosensing
Nanomaterials

Background:

Traditional electrochemical immunosensors face challenges with immune interference and steric hindrance.
Developing sensitive and efficient biosensing platforms for disease biomarkers is crucial.

Purpose of the Study:

To develop a novel split-type electrochemical immunosensor for sensitive neuron-specific enolase (NSE) detection.
To integrate a controlled-release strategy with electrochemical detection to minimize bioanalytical complexity and enhance sensitivity.

Main Methods:

Ascorbic acid (AA) was encapsulated in cadmium sulfide-capped spherical mesoporous bioactive glass nanocarriers (SBG_CdS).
A 96-well microplate was used for antibody-based immunoreactions, separating it from electrochemical detection.
AA was released using dithiothreitol, and its oxidation was catalyzed by a FcAI/l-Cys/gold nanoparticles/porous BiVO₄/ITO electrochemical sensing platform for signal amplification.

Main Results:

The electrochemical immunosensor demonstrated sensitive detection of neuron-specific enolase (NSE).
A wide linear range of 0.001-100 ng/mL and a low detection limit of 1.08 pg/mL were achieved.
The ordered nanoarray structure of porous BiVO₄ accelerated electron transfer, enhancing system sensitivity.

Conclusions:

The developed split-type electrochemical immunosensor effectively overcomes limitations of traditional methods.
This approach offers a sensitive and reliable method for neuron-specific enolase (NSE) biomarker assay.
The study presents an innovative strategy for the bioanalysis of NSE and potentially other biomarkers.