Profiling and Regulating Proteins That Adsorb to DNA Materials in Human Serum
Fan Xu1, Baijun Dong1, Xue Li1
1Institute of Molecular Medicine, Department of Urology, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, State Key Laboratory of Oncogenes and Related Genes, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.
Analytical Chemistry
|June 10, 2021
Summary
Researchers profiled proteins binding to DNA aptamers and nanostructures in human serum. They found distinct protein profiles and demonstrated a method to regulate these interactions for improved biological applications.
Area of Science:
- Biomaterials Science
- Proteomics
- Nanotechnology
Background:
- DNA aptamers and nanostructures have diverse biological applications.
- Protein adsorption to these DNA materials in physiological fluids impacts their function.
- Understanding and controlling protein interactions is crucial for optimizing DNA-based technologies.
Purpose of the Study:
- To quantitatively profile serum proteins that bind to DNA aptamers and nanostructures.
- To investigate the influence of serum origin (healthy vs. disease) on protein binding.
- To demonstrate strategies for regulating protein adsorption profiles.
Main Methods:
- Proteomic analysis using liquid chromatography-mass spectrometry (LC-MS).
- Characterization of protein binding to various DNA aptamers and nanostructures.
- Modification of DNA nanostructures to alter protein adsorption.
Main Results:
- Distinct protein profiles were identified for different DNA materials.
- Significant differences in protein binding were observed between healthy and cancer patient serum.
- Tethering single-stranded DNA onto nanostructures reduced complement protein adsorption and macrophage sequestration.
Conclusions:
- This study provides comprehensive proteomic profiles of serum proteins interacting with DNA materials.
- The findings highlight the potential to engineer DNA nanostructures for controlled protein interactions.
- Regulating protein adsorption can enhance the biological performance of DNA-based nanomedicines.
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