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Two-dimensional high-performance liquid chromatographic system for the direct determination of tocopherol derivatives
1Central Research Laboratory, Nisshin Flour Milling Co., Ltd., Saitama, Japan.
Abstract:
A procedure for the quantitative extraction of tocopherol derivatives from plasma was systematically developed using an efficient liquid-liquid distribution process. An aqueous stationary phase was coated on silica gel or diatomaceous earth powder in a closed-bed glass column prepared by slurry packing. A liquid-liquid chromatographic system provided with a detector was introduced as a preliminary extraction process. Liquid-solid chromatography followed as the second dimension of the separation and was used to determine alpha-tocopherol nicotinate in the extract. Stationary and mobile phases in the two-dimensional liquid chromatographic system were optimized to determine tocopherol derivatives. A highly sensitive quantitation method for alpha-tocopherol nicotinate in plasma was developed by using liquid-liquid and liquid-solid chromatography in conjunction with column switching by a tube-type micro-evaporator.