Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

7.4K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
7.4K
Proteomics01:33

Proteomics

8.7K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
8.7K
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

6.0K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
6.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A high-performance DC-AC signal separated photodetector for photothermal heterodyne imaging (PHI).

The Review of scientific instruments·2025
Same author

Development of an LC non-resonant detector for mid-infrared photothermal (MIP) microscopy.

The Review of scientific instruments·2025
Same author

Astigmatic Displacement Microscopy (ADM) with Subnanometer Axial Resolution for Three-Dimensional (3D) Morphological Characterization.

Analytical chemistry·2025
Same author

Droplet-Based Microfluidics in Single-Bacterium Analysis: Advancements in Cultivation, Detection, and Application.

Biosensors·2025
Same author

Tandem-repeat proteins introduce tuneable properties to engineered biomolecular condensates.

Chemical science·2025
Same author

Ganglioside lipids inhibit the aggregation of the Alzheimer's amyloid-β peptide.

RSC chemical biology·2025
Same journal

Microfluidic rare cell analysis beyond counting: workflow design from enrichment to multi-omics.

Lab on a chip·2026
Same journal

A sperm racetrack to separate sperm by swim speed.

Lab on a chip·2026
Same journal

Controlled encapsulation and droplet size prediction in two-step microfluidic double emulsions.

Lab on a chip·2026
Same journal

A particulate blood-mimicking fluid with physiological biconcave geometry for microscale hemorheology.

Lab on a chip·2026
Same journal

Multicellular sensor arrays fabricated by capillary stamping for pattern-based odor discrimination.

Lab on a chip·2026
Same journal

A real-time microfluidic surveillance system for multiplex detection of heavy metal contamination in wastewater.

Lab on a chip·2026
See all related articles

Related Experiment Video

Updated: Nov 2, 2025

An Integrated Approach for Microprotein Identification and Sequence Analysis
09:37

An Integrated Approach for Microprotein Identification and Sequence Analysis

Published on: July 12, 2022

3.6K

Machine learning-aided protein identification from multidimensional signatures.

Yuewen Zhang1, Maya A Wright1, Kadi L Saar2

  • 1Yusuf Hamied Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK. tpjk2@cam.ac.uk.

Lab on a Chip
|June 10, 2021
PubMed
Summary
This summary is machine-generated.

This study introduces a machine learning-assisted microfluidic method for protein identification. The technique generates unique 3D protein fingerprints, enabling accurate classification and direct identification from amino acid sequences.

More Related Videos

Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis
09:26

Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis

Published on: May 23, 2021

3.4K
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.3K

Related Experiment Videos

Last Updated: Nov 2, 2025

An Integrated Approach for Microprotein Identification and Sequence Analysis
09:37

An Integrated Approach for Microprotein Identification and Sequence Analysis

Published on: July 12, 2022

3.6K
Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis
09:26

Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis

Published on: May 23, 2021

3.4K
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.3K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Accurate protein identification is crucial for cellular biology and medical diagnostics.
  • Current methods for protein characterization can be time-consuming and complex.

Purpose of the Study:

  • To develop a rapid, microfluidic-based strategy for protein characterization and identification.
  • To leverage machine learning for classifying proteins based on their unique multidimensional fingerprints.

Main Methods:

  • A machine learning-aided microfluidic platform was developed to generate 3D protein fingerprints.
  • These fingerprints capture information on amino acid composition and size.
  • Machine learning algorithms were employed to classify ten different proteins based on their fingerprints.

Main Results:

  • The strategy achieved high accuracy in protein classification, outperforming single-dimension methods.
  • The generated 3D fingerprints showed a strong correlation with the amino acid content of the proteins.
  • Proteins could be identified directly from their sequence using the acquired fingerprints without prior data.

Conclusions:

  • This multidimensional profiling strategy offers a novel and efficient approach for protein identification.
  • The microfluidic format enables rapid characterization, with potential applications in diagnostics.
  • Direct protein identification from sequence data advances the field of proteomics.