Related Experiment Video
Updated: May 2, 2026
07:08
A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System
Published on: April 6, 2021
5.2K
REVEALR: A Multicomponent XNAzyme-Based Nucleic Acid Detection System for SARS-CoV-2
Journal of the American Chemical Society
|June 10, 2021
Summary
A new XNA-based platform, REVEALR, offers rapid and sensitive detection of COVID-19. This RNA-Encoded Viral Nucleic Acid Analyte Reporter system achieves high accuracy with a 1-hour assay time, providing a valuable tool for pathogen detection.
Area of Science:
- Biotechnology
- Molecular Biology
- Nucleic Acid Detection
Background:
- Isothermal amplification offers rapid, inexpensive, and accurate nucleic acid detection.
- Current methods for pathogen detection and disease diagnosis require improvement in speed and sensitivity.
Purpose of the Study:
- To develop a highly sensitive nucleic acid detection platform for the COVID-19 viral pathogen.
- To combine analyte preamplification with X10-23-mediated catalysis for enhanced detection.
Main Methods:
- Developed a multicomponent XNA-based platform named REVEALR (RNA-Encoded Viral Nucleic Acid Analyte Reporter).
- Utilized analyte preamplification and X10-23-mediated catalysis for detection.
- Employed conventional fluorescence and paper-based lateral flow readout modalities.
Main Results:
- Achieved a detection limit of ≤20 aM (∼10 copies/μL) for SARS-CoV-2.
- Demonstrated a total assay time of 1 hour.
- Showed no cross-reactivity with other respiratory viral RNAs and perfect accuracy with clinical samples.
Conclusions:
- REVEALR provides a sensitive, rapid, and accurate nucleic acid detection method for COVID-19.
- The platform serves as a convenient alternative to CRISPR-based approaches.
- REVEALR shows high specificity and accuracy for clinical diagnostics.
