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Viral Nanoparticles for In vivo Tumor Imaging

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Published on: November 16, 2012

Fusogenic Viral Protein-Based Near-Infrared Active Nanocarriers for Biomedical Imaging.

Suman Bishnoi¹, Anshu Kumari^1,2, Sheeba Rehman¹

¹Department of Biosciences and Biomedical Engineering, Indian Institute of Technology Indore, Indore 453552, India.

ACS Biomaterials Science & Engineering

|June 11, 2021

Summary

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Viral nanoparticles (VNPs) engineered with vesicular stomatitis virus glycoprotein (VSV-G) enhance cellular uptake and intracellular delivery of near-infrared (NIR) imaging agents. This study demonstrates their potential as effective drug delivery systems for improved bioimaging.

Area of Science:

Biotechnology
Nanomedicine
Molecular Imaging

Background:

Effective drug delivery systems (DDS) require efficient cellular uptake and intracellular delivery to prevent payload degradation.
Viral nanoparticles (VNPs) offer a natural mechanism for cell infiltration and material delivery.

Purpose of the Study:

To bioengineer vesicular stomatitis virus glycoprotein (VSV-G)-based viral nanoconstructs (NAVNs) for near-infrared (NIR) bioimaging.
To evaluate the cellular uptake and intracellular delivery efficiency of NAVNs encapsulating indocyanine green dye (ICG).

Main Methods:

Bioengineering of VSV-G based NAVNs encapsulating indocyanine green (ICG).
Assessment of NAVN size and cellular fusogenic properties.
NIR fluorescence imaging to compare cellular uptake of NAVNs versus free ICG.

Keywords:

ICG NAVNs NIR imaging VNPs VSV-G

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Main Results:

NAVNS exhibit spherical morphology and possess intrinsic cellular-fusogenic properties derived from VSV-G.
NIR imaging revealed significantly higher fluorescence intensity in cells treated with NAVNs compared to free ICG.
This indicates enhanced cellular uptake and intracellular delivery of ICG facilitated by NAVNs.

Conclusions:

VSV-G-based VNPs are effective nanoconstructs for drug delivery.
NAVNs demonstrate superior cellular uptake and intracellular delivery of NIR imaging agents.
The study validates the potential of VSV-G-based VNPs as efficient delivery systems for NIR fluorescence imaging applications.