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Nucleotide sequence of the gene encoding the matrix protein of a recent measles virus isolate
1Department of Biology, Queen's University of Belfast, U.K.
Abstract:
The sequence of the M gene of the Hu2 strain of measles virus has been determined. In the coding region of the gene, six nucleotide replacements had occurred with respect to the sequence of the M gene of the Edmonston strain. Two of these were silent and only four led to amino acid replacements in the protein sequence even though in SDS-PAGE the mobility of the protein would indicate an Mr increase of 2000. No changes were observed in the 5' untranslated regions of the gene. Two changes were observed in the 3' untranslated region of the gene. The sequences are compared to those recently published for subacute sclerosing panencephalitis viruses.
Insights
The measles virus M gene sequence was determined for the Hu2 strain. Minor genetic changes were found compared to the Edmonston strain, impacting protein structure and potentially disease mechanisms.
Area of Science:
- Virology
- Molecular Biology
- Genetics
Background:
- Measles virus (MeV) causes a significant global health burden.
- Understanding genetic variations in MeV strains is crucial for disease control and vaccine development.
- The matrix (M) protein plays a key role in MeV assembly and pathogenesis.
Purpose of the Study:
- To determine the complete nucleotide sequence of the M gene from the Hu2 strain of measles virus.
- To compare the M gene sequence of the Hu2 strain with that of the Edmonston strain.
- To analyze the implications of observed genetic variations on the M protein structure and function.
Main Methods:
- Nucleotide sequencing of the M gene from the Hu2 measles virus strain.
- Bioinformatic analysis to compare sequences with the Edmonston strain.
- Analysis of nucleotide and amino acid substitutions.
- Comparison with sequences from subacute sclerosing panencephalitis (SSPE) viruses.
Main Results:
- Six nucleotide replacements were identified in the M gene's coding region compared to the Edmonston strain.
- Two silent and four amino acid-altering substitutions were found, leading to a predicted Mr increase of 2000 for the M protein.
- No changes were observed in the 5' untranslated region, but two changes occurred in the 3' untranslated region.
- Sequence comparisons were made with published SSPE virus sequences.
Conclusions:
- The Hu2 strain M gene exhibits specific genetic variations compared to the Edmonston strain.
- These variations may influence M protein characteristics and potentially MeV pathogenesis.
- Further research is needed to elucidate the functional impact of these M gene alterations in measles and SSPE.