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Related Concept Videos

Proteomics01:33

Proteomics

8.7K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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DNA Microarrays02:34

DNA Microarrays

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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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Updated: Nov 2, 2025

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
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Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

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Protein Microarray-Based Proteomics for Disease Analysis.

Rodrigo Barderas1, Sanjeeva Srivastava2, Joshua LaBaer3

  • 1Chronic Disease Programme, UFIEC, Instituto de Salud Carlos III, Madrid, Spain. r.barderasm@isciii.es.

Methods in Molecular Biology (Clifton, N.J.)
|June 11, 2021
PubMed
Summary
This summary is machine-generated.

Protein microarrays are essential tools for understanding protein functions and their roles in disease. This book details methods for fabricating and applying protein microarrays to analyze protein interactions and responses for disease dissection.

Keywords:
Disease analysisFunctional analysisMicroarray data analysisProtein microarraysProtein scienceProteomics

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Area of Science:

  • Proteomics
  • Molecular Biology
  • Biotechnology

Background:

  • The Human Genome Project's completion highlights the need to understand protein functions.
  • Proteins are crucial for all aspects of biology and medicine.
  • Proteomics strategies are required to study gene products at scale.

Purpose of the Study:

  • To provide methodologies for fabricating protein microarrays.
  • To demonstrate applications of protein microarrays in disease analysis.
  • To dissect human disease by analyzing protein interactions and responses.

Main Methods:

  • Fabrication of protein microarrays.
  • Application of protein microarrays for various investigations.
  • Analysis of protein interactions and proteoform binding.

Main Results:

  • Protein microarrays enable examination of DNA, small molecule, and protein binding.
  • Characterization of humoral immune responses in health and disease.
  • Profiling of protein patterns as potential disease biomarkers.

Conclusions:

  • Protein microarrays are versatile tools for biological and medical research.
  • The methodologies presented aid in dissecting complex human diseases.
  • Advancing proteomics is key to understanding protein functions and disease mechanisms.