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Extended live-cell barcoding approach for multiplexed mass cytometry.

Muharrem Muftuoglu1, Li Li2, Shaoheng Liang3,4

  • 1Section of Molecular Hematology and Therapy, Department of Leukemia, The University of Texas MD Anderson Cancer Center, Unit 448, 1515 Holcombe Blvd., Houston, TX, 77030, USA.

Scientific Reports
|June 12, 2021
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Summary
This summary is machine-generated.

This study introduces a novel live-cell barcoding technique using cadmium (Cd) and palladium (Pd) tags for mass cytometry. This method enhances sample throughput and allows for greater pooling capacity in complex experiments.

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Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Immunology

Background:

  • Sample barcoding in mass cytometry improves throughput and reduces variability.
  • Live-cell barcoding is preferred over intracellular barcoding as it avoids fixation-dependent epitopes.
  • Current live-cell barcoding methods using palladium (Pd) isotopes have limitations in pooling capacity due to signal intensity and tag availability.

Purpose of the Study:

  • To develop a novel live-cell barcoding technique with enhanced sample pooling capacity.
  • To overcome the limitations of existing Pd-based barcoding methods.
  • To improve throughput and experimental flexibility in mass cytometry.

Main Methods:

  • Developed a new barcoding approach using a combination of seven cadmium (Cd) and three palladium (Pd) tags.
  • Utilized antibodies paired with these metal isotopes for live-cell barcoding.
  • Applied the technique to enable multiplexed sample processing and acquisition.

Main Results:

  • The novel technique employs 10 distinct tags (7 Cd, 3 Pd) with superior signal intensities.
  • Achieved enhanced sample pooling capabilities without compromising lanthanide detection.
  • Demonstrated a significant increase in sample throughput for mass cytometry experiments.

Conclusions:

  • The new Cd and Pd barcoding method significantly expands sample pooling capacity in mass cytometry.
  • This technique offers a robust solution for complex experimental designs and high-throughput analysis.
  • It represents a substantial advancement in live-cell barcoding for mass cytometry applications.