Synthesis and Characterization of Mitochondria-Targeted Triphenylphosphonium Bolaamphiphiles
Francesca Ceccacci1, Simona Sennato2, Edoardo Rossi3
1CNR-ISB, Sede Secondaria di Roma-Meccanismi di Reazione c/o Department of Chemistry, Sapienza University, Roma, Italy.
Abstract:
In this chapter we describe: (1) the procedure for the synthesis of four single chain bolaamphiphiles, displaying chains of 12, 16, 20 and 30 methylene units and triphenylphosphonium moieties as headgroups (TPP1-TPP4); (2) the methods used to characterize TPP1-TPP4 spontaneous aggregation in aqueous solution. We illustrate the determination of Krafft point and cac by conductivity measurements and the procedures used to investigate dimensions, morphology, and stability by dynamic and dielectrophoretic laser light scattering, dialysis, transmission electron microscopy, and Raman spectroscopy measurements.
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