Flexible electrochemical uric acid and glucose biosensor

Area of Science:

• Materials Science
• Electrochemistry
• Biosensors

Background:

• Developing integrated biosensors for simultaneous detection of biomarkers like uric acid (UA) and glucose is crucial for managing metabolic disorders.
• Existing biosensor platforms often face challenges in sensitivity, selectivity, and integration for practical applications.

Purpose of the Study:

• To fabricate fully integrated uric acid and glucose biosensors on a polydimethylsiloxane/polyimide platform.
• To enhance the electrochemical activity and performance of the biosensors through surface functionalization and nanoparticle decoration.

Main Methods:

• Utilized a facile one-step laser-scribed technique to create laser-scribed graphene (LSG) on a polyimide film.
• Functionalized LSG with pyrenebutanoic acid, succinimide ester (PBSE) and decorated with platinum nanoparticles (PtNPs).
• Immobilized glucose oxidase for glucose detection and characterized the biosensors using SEM, EDX, XPS, cyclic voltammetry, and amperometry.

Main Results:

• Achieved high sensitivity for uric acid detection (156.56 µA/mMcm²) within a wide range (5–480 µM) and a low detection limit (0.018 µM).
• Demonstrated excellent performance for glucose sensing with a broad detection range (5–3200 µM), sensitivity of 12.64 µA/mMcm², and a detection limit of 2.57 µM.
• Confirmed outstanding electrocatalytic activities and good selectivity, sensitivity, and stability properties of the integrated biosensors.

Conclusions:

• The developed integrated biosensors exhibit significant potential for wearable applications in real-time uric acid and glucose monitoring.
• The facile fabrication method and enhanced electrochemical properties pave the way for advanced diagnostic tools.
• These biosensors offer a promising platform for personalized health management and early disease detection.