Time-dependent deterioration of active transport in duodenal segments of rat intestine

In this study, a time-dependent deterioration of the active transport in duodenal segments of rat intestine was observed. The everted gut-sac technique was used to quantitate both calcium and glucose transport in intestinal segments. The results indicate that both calcium and glucose transport decreased significantly in intestinal segments of animals killed by cervical dislocation 10-20 min prior to tissue removal. It was further determined that animal anesthesia permitted excision of intestinal segments prior to death and thus avoided transport deterioration.