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Molecular identification, virulence factors and antifungal susceptibility patterns of Candida parapsilosis complex

N Cakir1, M A Atalay1, A N Koc1

  • 1Department of Medical Microbiology, School of Medicine, Erciyes University, Kayseri, Turkey.

Nigerian Journal of Clinical Practice
|June 14, 2021
PubMed
Abstract

Insights

Candida parapsilosis sensu stricto (CpSS) is the most common species in the C. parapsilosis complex. Molecular identification of CpSS is not essential for routine labs due to similar antifungal susceptibility across species.

Area of Science:

  • Medical Mycology
  • Clinical Microbiology
  • Molecular Diagnostics

Background:

  • The Candida parapsilosis complex is a significant cause of fungal infections.
  • Accurate identification of species within this complex is crucial for understanding pathogenicity and guiding treatment.
  • Virulence factors and antifungal susceptibility can vary among Candida species.

Purpose of the Study:

  • To identify Candida parapsilosis complex strains using sequence analysis.
  • To compare virulence factors and antifungal susceptibility between different species within the complex.
  • To evaluate the utility of molecular methods for routine identification.

Main Methods:

  • 42 Candida parapsilosis complex isolates were identified using chromogenic medium, morphology, and API 20 C AUX.
  • DNA sequence analysis of internal transcribed spacers (ITS1 and ITS4) was performed.
  • Virulence factors (biofilm, esterase, phospholipase, protease) and antifungal susceptibility were assessed.

Main Results:

  • 41 of 42 isolates were identified as Candida parapsilosis sensu stricto (CpSS), and one as Candida orthopsilosis.
  • CpSS strains frequently exhibited biofilm, esterase, phospholipase, and protease activities.
  • All CpSS strains were susceptible to amphotericin B, echinocandins, and flucytosine; C. orthopsilosis showed no virulence factors.

Conclusions:

  • Candida parapsilosis sensu stricto is the predominant species in the C. parapsilosis complex.
  • Molecular identification of CpSS may not be necessary for routine diagnostics due to consistent antifungal susceptibility profiles.
  • No significant differences in antifungal susceptibility were observed between the identified species.
Keywords:
Antifungal susceptibilityC. metapsilosisC. orthopsilosisC. parapsilosis sensu strictoCandida's parapsilosis complexvirulence

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